Promotive Communications

Lab Focus AugustSept digital

Laboratory Focus is Canada's leading editorial-based lab publication. Providing readers with the latest technology updates through application and tech notes, as well as covering new products and trends in laboratories across Canada.

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R&D News ......................... 1 Appointments .................... 6 Pharma Notes .................... 7 New Products .................. 14 Calendar .......................... 17 App Reviews ..................... 18 P h a r m a c e u t i c a l c l i n i c a l c h e m i c a l f o o d e n v i r o n m e n t w w w . l a b o r a t o r y f o c u s . c a august/sePtember 2014 volume 18, number 3 Publications Mail Registration Number: 40052410 tak mak study in cancer cell maPs decade of discovery to Potential anticancer agent claimed immunologist renowned for his 1984 cloning of the genes encoding the human T-cell receptor. In the lab, Dr. Mak`s scientific team used an approach that com- bined functional RNAi analysis with gene expression analysis in breast cancer-derived cell lines and in human breast cancers replicated in mice. Using these multidimen- sional data sets for human breast cancer, PLK4 was identified as a candidate target among 10,000 other targets for the development of anticancer therapeutics. "The research showed that the aggressive form of basal breast cancer cells may be dependent on PLK4 for survival and that depleting it induced cell death," says Dr. Mak. "This finding led to the identification of CFI-400945, a selective and orally active inhibitor of PLK4, which was shown to have significant anti-tumour activity as a single agent in a variety of preclini- cal tumour models." Another key finding was observ- ing the inhibitor effect on tumour models with a gene PTEN deficien- cy as a biomarker – of huge interest because PTEN, a tumour suppres- sor, is known to be defective in as many as half of all advanced solid tumour cancers. "If clinical testing supports our hypothesis that PTEN is a predictive biomarker for CFI-40095, we will have another way to tailor personal- ized cancer medicine based on an individual's genetics," he says. Although breast cancer was the initial focus and featured in the study that was published, the team has also conducted experiments with other types of solid tumours, with similar results. The next phase of research will involve testing in humans, which was approved last year by Health Canada and the U.S. Food and Drug Administration. "It may take several more years to determine the benefit for patients," says Dr. Mak, "but we are happy to be able to provide this opportunity for our patients. We remain opti- mistic that we may have found a novel way to treat cancer." The research was funded by The Princess Margaret Cancer Foun- dation, the Canadian Institutes of Health Research and Genome Canada. To see this story online visit http://www.laboratoryfocus. ca/?p=2490 The journal Cancer Cell has just published research led by Dr. Tak Mak mapping the path of discovery to developing a potential anticancer agent. "What began with the question 'what makes a particular aggressive form of breast cancer cells keep growing?' turned into 10 years of systematic research to identify the enzyme PLK4 as a promising thera- peutic target and develop a small molecule inhibitor to block it," says Dr. Mak, director of The Campbell Family Institute for Breast Cancer Research at the Princess Margaret Cancer Centre, University Health Network and an internationally ac- measuring safety Performance Page 11 laboratory equipment: What to Purchase Page 12 Dr. Tak Mak

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